Evaluation of Taxifolin and Phloretin as Antiangiogenic Flavonoids: an in Vivo, in Vitro Experimental Analysis

Objective: To examine the antiangiogenic effect of some flavonoids (taxifolin and phloretin) against various In vivo and In vitro experimental models such as Chick Chorio Allantoic Membrane (CAM) assay, Dorsal Skinfold Chamber (DSFC) and Tube formation assay. Methods: Three different concentrations of drug solution (40μM, 80 μM, 120 μM) and (50 μM, 100 μM, 150 μM) were applied topically during CAM and DSFC assay. These assays were performed to detect antiangiogenic effect of the selected molecules. In tube formation assay, vascular endothelial growth factor stimulated Human Umbelical Vain Endothelial Cells (HUVECs). Results: Antiangiogenic results showed that different concentration of taxifolin and phloretin inhibited new blood vessels and vessels branches per area of CAMs. Similarly, there are reduced numbers and diameter of blood vessels when the doses of the drug increased which are observed in different experimental groups in DSFC model. In vitro anti angiogenic effect of our molecules was further confirmed by inhibition of tube formation on matrigel matrix that involved HUVECs. All the test groups were compared with the control group. Conclusion: The data reported here provide evidence that flavonoids taxifolin and phloretin revealed prominent anti angiogenic activities.